-
BUDGET SAVER — Save $50 on every ELISA kit. Limited-time deal.
Overview
-
Vector Type
Mammalian Cells / Lentivirus -
Insert Type
ORF -
Target Species
Rat -
Promoter
CMV -
Selection Marker
Puromycin -
Copy Number
High
| Catalog Number | BV-P10001 |
| Bacterial Resistance | Ampicillin |
| Mammalian Selection Marker | Puromycin |
| Reporter / Fluorescent Marker | Green |
| Origin of Replication | pUC |
| Copy Number (E. coli) | High |
| Competent Cell Strain | Stbl3 |
| Recommended Growth Temperature | 37°C |
| Backbone Vector | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
| Target Sequence / Alias | NM_053304.1;COLIA1 |
| Forward Primer | CMV-F |
| Insert Length | 4359 |
| Total Plasmid Length | 12649 bp |
Full Sequence
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta
2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt tcagctttgt ggacctccgg
2341 ctcctgctcc tcttaggggc cactgccctc ctgacgcatg gccaagaaga catccctgaa
2401 gtcagctgca tacacaatgg cctaagggtc cctaatggtg agacgtggaa acctgatgta
2461 tgcttgatct gtatctgcca caatggcacg gctgtgtgcg atggcgtgct atgcaaagaa
2521 gacttggact gtcccaaccc ccaaaaacgg gagggcgagt gctgtccttt ctgcccagaa
2581 gaatatgtat caccagacgc agaagtcata ggagtcgagg gacccaaggg agaccctggc
2641 ccccaaggcc cacggggacc tgttggcccc cctggacaag atggcatccc tggacagcct
2701 ggacttcctg gtcctcctgg tccccccggc ccccccggac cccctggtct tggaggaaac
2761 tttgcttccc agatgtccta tggctatgat gagaaatcag ctggagtttc cgtgcctggc
2821 cccatgggtc cttctggtcc tcgtggtctc cctggccccc ctggtgcacc tggtcctcaa
2881 ggtttccaag gcccccctgg tgaacctggc gagcctggcg cttcaggtcc aatgggtccc
2941 cgaggtcccc ctggccctcc tggcaagaac ggagatgatg gggaagctgg taagcctggc
3001 cgccctggtg agcgtggacc tcctggacct cagggtgctc gtggattgcc tggaacagct
3061 ggcctccccg gaatgaaggg acacagaggt ttcagtggtt tggatggtgc caaaggagat
3121 actggtcctg ctggtcctaa gggagagcct ggcagtcctg gtgaaaatgg agctcccggc
3181 cagatgggtc cccgaggtct gcctggtgag agaggtcgcc ctggaccccc tggcagtgct
3241 ggtgctcgtg gtaacgatgg tgctgtcggt gcagctgggc cccccggtcc caccggccct
3301 actggccctc ctggcttccc tggtgcagct ggtgctaagg gtgaagctgg tccccaggga
3361 gcccgaggct ctgaaggtcc ccagggtgtg cgtggtgagc ccggaccccc tggccctgct
3421 ggtgctgctg gtcctgctgg aaaccctggt gctgatggac aacctggtgc taaaggtgcc
3481 aatggtgctc ctggtattgc tggtgctcct ggcttccctg gtgcccgagg cccctctgga
3541 cctcagggcc ccagcggcgc tcctggtccc aaaggcaaca gtggtgaacc tggtgcccct
3601 ggcaacaaag gagacactgg tgccaaagga gaacccggcc ctgctggagt tcaaggtccc
3661 cctggccctg ctggagaaga aggaaaacga ggagcccgtg gtgagcctgg acctagcggc
3721 ctgcccggac ctcctggcga gcgcggtgga cctggtagcc gtggtttccc tggtgctgat
3781 ggtgttgctg gccccaaggg tcctgctggt gaacgtggtt ctcctggccc tgctggtccc
3841 aaaggttctc ctggtgaagc tggtcgccct ggtgaagctg gtctccctgg tgccaagggt
3901 ctcactggca gtcctggcag ccctggtcct gatggcaaaa ccggcccccc tggtcccgct
3961 ggtcaagatg gtcgccctgg acccgcaggt cctcctggag cccgtggaca ggctggtgtg
4021 atgggattcc ctggacctaa gggtactgct ggagaacctg gaaaggctgg agaacgaggt
4081 gtccccggac cccctggcgc tgttggtccc gctggcaaag atggcgaagc tggagctcaa
4141 ggagcccccg gccctgctgg tcctgctggt gagagaggtg aacaaggtcc cgctggctcc
4201 cctggattcc agggtcttcc tggtcccgct ggtcctcctg gtgaagcagg caagcctggt
4261 gaacagggtg ttcctggaga ccttggtgcc cctggaccct ctggcgcaag aggcgagaga
4321 ggtttccctg gtgaacgtgg tgtacaaggt cccccaggtc ctgctggtcc ccgaggaaac
4381 aatggtgccc ccggcaacga tggtgccaag ggtgatactg gtgcccccgg agctcctggt
4441 agccagggtg cccccggtct tcagggaatg cctggtgaac gtggtgcagc tggtcttcca
4501 ggtcctaagg gtgacagagg tgatgctggt cccaaaggtg ctgatggttc tcctggcaaa
4561 gatggcgtcc gtggtctgac tggtcccatt ggtcctcctg gccctgctgg tgcccctggt
4621 gacaagggtg aaactggtcc cagtggtcct gctggcccca ccggagcccg tggtgccccc
4681 ggagaccgtg gtgagcctgg tccccctggt cctgctggct tcgctggccc ccctggtgct
4741 gatggccaac ctggtgcgaa aggtgaacct ggtgatactg gtgtgaaagg tgacgctggt
4801 cctcctggcc ctgctggtcc cgctggaccc cctggcccca ttggtaacgt tggtgctcct
4861 ggacccaaag gttctcgtgg tgctgctggt ccccctggtg ctactggttt ccctggtgct
4921 gctggtcgtg ttggtccccc tggtccctct ggaaatgctg gaccccctgg ccctcccggt
4981 cccgttggca aagaaggggg caaaggtccc cgtggtgaga ctggtcccgc tggacgtcct
5041 ggtgaagttg gtcccccagg tccccctggc cctgctggtg agaaaggatc tcctggtgct
5101 gatggacctg ctggctctcc tggtacccct ggacctcagg gtattgctgg acagcgtggt
5161 gtggtcggtc ttcccggtca gagaggagaa agaggcttcc ctggtcttcc tggaccctct
5221 ggtgaacccg gcaaacaagg tccttctgga gcaagtggtg aacgtggtcc ccctggccct
5281 atgggccccc ctggattggc tggcccccct ggtgaatctg gtcgtgaggg atcccctggt
5341 gctgaaggct cccctggaag agatggtgct cctggtgcca agggtgaccg tggtgagact
5401 ggccctgctg gcccccctgg tgctcctggt gctcctggtg ctcccggccc tgttggtcct
5461 gctggcaaga atggcgaccg tggtgagact ggtcctgctg gtcctgctgg tcccattggc
5521 cctgctggtg cccgtggtcc tgctggaccc caaggccccc gtggtgacaa gggtgagaca
5581 ggcgaacaag gtgacagagg cataaagggt catcgtggct tctctggtct ccagggtcct
5641 cctggctctc ctggctctcc tggtgaacaa ggcccctctg gagcttctgg tcctgcaggt
5701 ccccggggtc cccctggctc tgctggttct cctggcaaag atggactcaa cggtctccct
5761 ggccccattg gtccccctgg tcctcgaggt cgcactggcg atagtggtcc tgctggtccc
5821 cccggacctc ctggaccccc tggccctccc ggtcctccca gcggtggtta tgacttcagc
5881 ttcctgcctc agccacctca agagaagtct caagatggtg gccgttacta ccgggccgat
5941 gatgccaacg tggtccgtga ccgtgacctt gaggtggaca ctaccctcaa gagcctgagc
6001 cagcagattg agaacatccg cagccctgag ggcagccgca agaaccccgc ccgcacatgc
6061 cgtgacctca agatgtgcca ctctgactgg aagagcggag agtactggat cgaccctaac
6121 caaggctgca acctggatgc catcaaggtc tactgcaaca tggagacagg tcagacctgt
6181 gtgttcccca ctcagccctc tgtgcctcag aagaactggt acatcagccc aaaccccaag
6241 gagaagaagc atgtctggtt tggagagagc atgaccgatg gattccagtt cgagtatgga
6301 agcgaaggtt ccgatcctgc cgatgtcgct atccagctga ccttcctgcg cctgatgtcc
6361 accgaggcct cccagaacat cacctatcac tgcaagaaca gcgtagccta catggaccaa
6421 cagactggca acctcaagaa gtccctgctc ctccagggct ccaacgagat cgagctcagg
6481 ggcgaaggca acagtcgatt cacctacagc acgcttgtgg atggctgcac gagtcacacc
6541 ggaacttggg gcaagacagt catcgaatac aaaaccacca agacctcccg cctgcccatc
6601 atcgatgtgg ctcccttgga cattggtgcc ccagaccagg aattcggaat ggacattggc
6661 cctgcctgct tcgtgggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC
6721 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt
6781 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
6841 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
6901 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
6961 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
7021 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
7081 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
7141 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
7201 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
7261 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
7321 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
7381 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
7441 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
7501 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
7561 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
7621 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
7681 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
7741 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
7801 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
7861 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
7921 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
7981 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
8041 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
8101 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
8161 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
8221 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
8281 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
8341 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
8401 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
8461 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
8521 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
8581 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
8641 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
8701 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
8761 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
8821 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
8881 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
8941 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
9001 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
9061 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
9121 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
9181 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
9241 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt
9301 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
9361 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata
9421 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
9481 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
9541 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
9601 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
9661 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
9721 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
9781 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG
9841 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
9901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
9961 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
10021 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
10081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
10141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
10201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
10261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
10321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
10381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
10441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
10501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
10561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
10621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
10681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
10741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
10801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
10861 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
10921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
10981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
11041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
11101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
11161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
11221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
11281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
11341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
11401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
11461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
11521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
11581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
11641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
11701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
11761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
11821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
11881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
11941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
12001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
12061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
12121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
12181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
12241 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
12301 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
12361 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
12421 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
12481 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
12541 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
12601 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctgDon't See the Construct You Want?
Let us do the heavy lifting. We offer expert sourcing for hard-to-find clones and fully customizable antibody solutions to perfectly align with your research goals.
Talk to Our Experts
Related Products
-
FROM $95.00
-
FROM $65.00
-
FROM $65.00
-
FROM $80.00
-
FROM $65.00
-
FROM $245.00
