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Molecular Cloning Services
Custom DNA Constructs, Viral & Non-Viral Vectors100% sequence-accurate custom cloning for any vector type — plasmid, AAV, lentivirus, adenovirus or other backbones. From a simple subclone to a complex multi-fragment assembly or full viral packaging, BrainVTA delivers ready-to-use constructs faster and more cost-effectively than in-house cloning.
Request a Quote
Online Inquiry
Request a Quote
Send your gene, sequence or map — a vector scientist replies within one business day.
- 100% sequence-verified
- Plasmid or packaged virus
- CoA + sequence files
Request a Quote
Online Inquiry
AT A GLANCE
- Sequence accuracy 100% (Sanger)
- Turnaround From 5 days
- Subcloning, <1 kb $160
- Gene synthesis $0.17 / bp
- Backbones 200+ / your own
- Packaging AAV · LV · AdV
Full-Spectrum Molecular Cloning
BrainVTA offers a complete range of molecular cloning services to build custom DNA constructs for any downstream application. Provide your project information — gene name, species or sequence — and we deliver ready-to-use plasmid or viral particles.
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100% Sequence Accuracy
Verified by Sanger sequencing.
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Fast Turnaround
From 5 business days for standard subcloning.
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Flexible Options
Viral or non-viral, any promoter, tag or reporter.
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Cost-Effective
Save reagents, labour and frustration vs DIY cloning.
Key Advantages
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Validated Backbones
Higher efficiency, proven results.
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Ready-to-Use Templates
Fast modular swapping.
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Flexible Subcloning
Adapt to any vector or fragment.
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One-Stop Service
From clone to protein or viral particles.
Core Cloning Services
We cover common and advanced cloning needs, including but not limited to:
| SERVICE | DESCRIPTION |
|---|---|
| Standard subcloning | Transfer your insert into any plasmid vector. |
| Site-directed mutagenesis | Point mutation, deletion or insertion — single or multiple sites. |
| Gene synthesis | Synthetic genes with codon optimization, any length or sequence. |
| Multi-fragment assembly | Seamless assembly of up to 10 fragments and more (Gibson / In-Fusion). |
| shRNA / siRNA vectors | U6/H1 or miR30-based shRNA expression vectors (≥70% knockdown guaranteed). |
| CRISPR-Cas9 vectors | gRNA expression vectors for knockout / knock-in. |
| Viral vector cloning | Cloning into AAV, lentivirus (LV) or adenovirus (AdV) transfer plasmids. |
| Non-viral vector cloning | Transient transfection, piggyBac / Sleeping Beauty transposons, bacterial expression. |
| Custom library construction | gRNA libraries, ORF libraries, mutagenesis libraries. |
Custom Vector Subcloning
Already have a unique or commercially restricted backbone? We can subclone into your own vector.
Start Your Project
Pricing & Turnaround
Standard subcloning and gene synthesis pricing is listed below. Final pricing may vary with vector complexity, repetitive sequences, GC content and other structural features.
| Insert size | Timeline | Price (USD) |
|---|---|---|
| <1kb | 1-2 weeks | $160.00 |
| 1-2 kb | 1-2 weeks | $183.00 |
| 2-3 kb | 1-2 weeks | $283.00 |
| 3-4 kb | 1-2 weeks | $416.00 |
| >4kb | Inquire | Inquire |
Add-On Service
If gene synthesis is needed, additional price and turnaround time for vector construction services:
| Add-on service | Timeline | Price (USD) |
|---|---|---|
| Gene Synthesis | 1-2 weeks | $0.17 / bp |
Workflow- Four Core Steps
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1
Choose a Vector Backbone
Select a viral or non-viral vector for your application.
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2
Choose a Promoter
Tissue-specific, constitutive or inducible expression.
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3
Add the Gene of Interest, Tags, Reporters & Linkers
Select a viral or non-viral vector for your application.
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4
Clone → Sequence → Deliver
Ready-to-use plasmid delivery or viral packaging.
Step 1 — Choosing a Vector Backbone
Viral Vector Comparison
| Property | AAV | LV | Adenovirus |
|---|---|---|---|
| Genome | ssDNA | ssRNA (+) | dsDNA |
| Envelope | Naked | Enveloped | Naked |
| Type | Non-integrating | Integrating | Non-integrating |
| Infected Cells | Dividing & non‑dividing | Dividing & non‑dividing | Dividing & non‑dividing |
| Packaging Capacity | ≤4.9 kb | ≤6kb | ≤7.5kb |
| Expression Duration | Long‑lasting (non‑integrating but stable) |
Long‑lasting (integrated) |
Transient (high level) |
| Immunogenicity | Very Low | Low | High |
| Transduction Efficiency | Moderate | Moderate | High |
| Biosafety | BSL-1 | BSL-2 | BSL-2 |
Common Non-viral Vectors
| Vector type | Application | Capacity limit |
|---|---|---|
| pCDNA3.1(+) | Mammalian transient expression | No strict limit (>10 kb possible) |
| pEGFP-N1/C1 | Fusion protein + GFP tracking | No strict limit |
| pUC19 / pBR322 | Routine subcloning, amplification | No strict limit |
| pET series | Protein expression in E. coli | No strict limit |
| piggyBac / Sleeping Beauty | Transposon mediated stable integration | Large fragments possible |
We also support your own backbone (map and resistance info required), or choose from our library of >200 commonly used vectors.
Step 2 — Choose a Promoter
Constitutive / Ubiquitous Promoters
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Mammalian
CMV, CAG, EF1α, PGK, UBC
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Prokaryotic
T7, lac, araBAD
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Yeast
ADH1, GAL1
Tissue-Specific Promoters
Don't see the promoter you need? Many more are in our library, and custom promoter / serotype services are available.
Step 3 — Adding GOI, Tags, Reporters & Linkers
BrainVTA maintains >20,000 pre-cloned genes (human, mouse, rat and other models). Combine elements freely:
| Element | Options |
|---|---|
| Protein Tags | 6xHis, 3XFlag,HA, Myc |
| Subcellular Localization Signals |
H2B(nuclear),NLS(nuclear), soma(cell body), NES (nuclear export), LCK(membrane), mito(mitochondria), axon |
| Linkers | 2A peptide (self‑cleaving), IRES (internal ribosome entry site) |
| Reporters | EGFP, mCherry, RFP,tagBFP, CFP, mCelulean, mCitrine, dsRed, Luciferase,ect |
If your GOI isn't in our pool, we can synthesize the gene, design shRNA / CRISPR, perform site-directed mutagenesis, or PCR-amplify and subclone from your template.
Step 4 — Cloning, Validation & Delivery
Cloning Methods
| Method | Best for | Features |
|---|---|---|
| Restriction-Ligation | Single fragment, routine subcloning | Classic, stable – requires compatible sites |
| Gibson Assembly | 2-10 fragments, seamless | No restriction sites needed |
| In-Fusion | Single or multiple fragments | Seamless, good for long fragments |
| TOPO / TA Cloning | Quick entry of PCR products | Very fast (5 min ligation) |
| Gateway Recombination | Shuttling into multiple vectors | High-throughput compatible |
Quality Control & Deliverables
Every plasmid is verified by full-insert double-strand Sanger sequencing (report provided) and restriction mapping (gel image with expected sizes). You receive plasmid DNA in solution, a glycerol stock (DH5α or Stbl3), sequence files and a Certificate of Analysis. Custom constructs can be packaged into AAV, lentivirus or adenovirus on request.
Frequently Asked Questions
Which bacterial strains can I use to propagate my plasmid?
We commonly use DH5α and Stbl3, and your plasmid can be propagated in either.
Are there limits to which ORFs can be shuttled?
We commonly use DH5α and Stbl3, and your plasmid can be propagated in either.
Related Services
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Knockdown Stable Cell LineshRNA-integrated stable lines for durable, heritable knockdown.Learn More -
Overexpression Cell LineStable lines durably expressing your gene of interest, single- or multi-copy.Learn More
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Point Mutation Cell LinePrecise single-nucleotide edits by HDR or base editing, sequence-confirmed.Learn More
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Knock-In Cell LineTargeted insertion of tags, reporters or edits at the endogenous locus.Learn More
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Virus-Like Particles (VLPs)Non-replicating particles for delivery, vaccine development and assay work.Learn More
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Adenovirus PackagingHigh-level transient expression with adenoviral vectors up to 7.5 kb capacity.Learn More
